Reduced thrombus formation in f10^low mice in laser–induced vessel injury model. A localized laser injury was induced in the cremaster arterioles of f10^WT (n = 4) or f10^low (n = 4) mice. Clot formation was visualized by monitoring the accumulation of platelets and the deposition of fibrin. Platelets (red) and fibrin (green) were detected using AlexaFluor647-conjugated anti-GPIb and Alexa Fluor 488-conjugated antifibrin antibodies, respectively. (A) Representative digital composite fluorescence and bright-field images of platelet accumulation (red) and fibrin deposition (green) in f10^WT (n = 46 clots) and f10^low (n = 44 clots) mice before (0 s) and 20, 80, 120, 200, and 300 s after laser injury. (B,D) Kinetic curves displaying platelet accumulation and fibrin deposition in f10^WT (blue; 46 clots) and f10^low (red; 44 clots) mice. Plotted is median IFI for platelets (B) and fibrin (D) fluorescence vs time after injury. (C,E) Distribution of the integrated platelet (C) or fibrin (E) fluorescence intensity for each of the 46 clots in f10^WT mice and 44 clots in f10^low mice. Plotted is the AUC for each individual thrombus. Presented are Box-Whisker plots with minimum to maximum values, with all individual data points shown. Statistical analysis was performed using the Mann-Whitney test. AUC, area under the curve; IFI, integrated fluorescence intensity.