Figure 2.
Characterization of EBV lytic transcripts in EBV+ HL. Comprehensive EBV gene transcription analysis was performed in complementary DNA generated from RNA isolated from EBV+ HL biopsy samples. (A) Scatterplot showing the cycle threshold (Ct) value produced by transcriptome profiling of EBV genes by real-time qualitative polymerase chain reaction. Higher CT values indicate fewer EBV transcripts. EBV+ lymphoma biopsy samples are denoted by red symbols (n = 6), and EBV-negative lymphoma biopsy samples (n = 2) are shown in black. The Namalwa cell line used as positive control is shown in green. The Ct value cutoff for the absence of EBV transcripts is set at 35 based on previous work.45