Differential abundance and gene expression in primary MDS cells. (A) Primary cells in UMAP representation overlaid with density plot for differential abundance in MDS vs HY cohort (see also supplemental Figures 5A and 6). (B) Barplot of frequency per cell population for n = 11 580 HY and 12 791 MDS cells. (C) Density histogram of pseudotime estimate for n = 2 selected cell populations separated by sample origin. Dashed lines indicate the median per cell population in condition, 2-sided t test adjusted using Benjamini-Hochberg procedure. (D) Gene expression along erythroid pseudotime differentiation for subset of n = 9168 cells designated to erythropoiesis of averaged gene expression using (AddModuleScore) for IL1 target genes according to gene ontology (GO:0070498). Gray shades represent standard error. (E) Progeny analysis for pathway activation in erythropoietic cells. (F) Gene expression along myeloid pseudotime differentiation for subset of n = 9064 cells designated to myelopoiesis, an averaged gene expression using for leucocyte migration–related genes according Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (hsa04670, top), and cyclin dependent kinase 6, (bottom). (G) Cell cycle analysis derived from transcriptomic data for myeloid progenitor cells groups per cell population. CMP, common myleoid progenitor; GMP, granulocyte/monocyte progenitor.