TP53 mutations in primary and acquired resistance to InO. (A) Baseline genomic alterations in major leukemia genes, comparing responders and nonresponders. (B) EFS and overall survival in TP53WT vs TP53mut patients. The elapsed observation time is plotted on the curve as a circle. Symbols for both events and censored observations were plotted so that each subject was shown. The censored observations can be clearly seen as circles along the horizontal portion of the curve. The medians were estimated using the Kaplan-Meier method. P values were determined using the log-rank test. (C) Dynamic changes in TP53 VAF in 5 cases with paired pre- and post-InO samples. The VAF for each mutation is shown along with the sample blast count at the corresponding time point. The time elapsed from the start of InO to treatment failure (R/R) was is in days. CI, confidence interval; HR, hazard ratio; mut, mutation; NGS; next-generation sequencing; WES, whole-exome sequencing; WGS, whole-genome sequencing; WT, wild-type.