Platelet-associated TGF-β1 affected the TGF-β/Smad pathway in active-ITP mice. Immunofluorescence images of femurs from control and Romi-treated mice. (A) MFI of Ly6C/6G in live cells and Smad2 in Gr-1⁺ cells. (B) MFI of Ly6C/6G in live cells, and p-Smad2 in Gr-1⁺ cells. (C) MFI of Ly6C/6G in live cells and Smad3 in Gr-1⁺ cells. (D) MFI of Ly6C/6G in live cells and p-Smad3 in Gr-1⁺ cells.