Smc3 haploinsufficiency partially restores HSC quiescence in Yy1^−/−mice. (A) Representative gating strategy for Ki67/DAPI cell proliferation assay of LT-HSCs (Lin⁻Sca1⁺c-Kit⁺CD48⁻CD150⁺). Cells in the G0 phase were defined as Ki67⁻DAPI⁻. Cells in the G1 phase were defined as Ki67⁺DAPI⁻. Cells in S/G2/M phase were defined as Ki67⁺DAPI⁺. (B-D) Quantification of percentages of LT-HSC, ST-HSC, and MPP cells in G0, G1, and S/G2/M phase. (E) Experimental strategy of BM transplantation (BMT). (F) Quantification of donor-derived contribution in B-cell (Thy1.2⁻CD19⁺), T-cell (Thy1.2⁺CD19⁻), monocyte (Mac1⁺Gr1⁻), and neutrophil (Mac1⁺Gr1⁺) populations at 4, 8, 12, 16, and 20 weeks after BMT. N represents the number of mice; data are presented as means ± SD; ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.