Figure 5.
Smc3 haploinsufficiency partially rescues Yy1−/− HSPC percentages and numbers. (A) SMC3 expression was normalized in Yy1−/−Smc3+/− BMCs. Western blot and quantification to detect SMC3 and YY1 protein expressions in BMCs of Yy1−/−, Yy1+/+, Smc3+/−, and Yy1−/−Smc3+/− mice. (B) Survival curve of Yy1+/+, Yy1−/−, Smc3+/−, and Yy1−/−Smc3+/− mice. (C) Complete blood count (CBC) analysis. (D) Representative flow gating strategy for LSK, LT-HSC, ST-HSC, MPP, MP, common myeloid progenitors (CMP), granulocyte monocyte progenitors (GMP), and megakaryocyte erythroid progenitors (MEP) populations. (E) Quantification of percentages and absolute numbers of LSK, LT-HSC, ST-HSC, MPP, MP, CMP, GMP and MEP populations. N represents the number of mice; data are presented as means ± SD; ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.

Smc3 haploinsufficiency partially rescues Yy1−/− HSPC percentages and numbers. (A) SMC3 expression was normalized in Yy1−/−Smc3+/− BMCs. Western blot and quantification to detect SMC3 and YY1 protein expressions in BMCs of Yy1−/−, Yy1+/+, Smc3+/−, and Yy1−/−Smc3+/− mice. (B) Survival curve of Yy1+/+, Yy1−/−, Smc3+/−, and Yy1−/−Smc3+/− mice. (C) Complete blood count (CBC) analysis. (D) Representative flow gating strategy for LSK, LT-HSC, ST-HSC, MPP, MP, common myeloid progenitors (CMP), granulocyte monocyte progenitors (GMP), and megakaryocyte erythroid progenitors (MEP) populations. (E) Quantification of percentages and absolute numbers of LSK, LT-HSC, ST-HSC, MPP, MP, CMP, GMP and MEP populations. N represents the number of mice; data are presented as means ± SD; ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.

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