Sustained changes in B- and T-cell subsets. (A) Individual and mean senescent (CD57⁺) CD4⁺ T-cell percentages over time. n values for part 1 baseline, Ext Scr, and years 1, 2, 3, 4, 5, and 6 are as follows: 6, 6, 4, 1, 3, 2, 1, and 3, respectively. (B) Individual and mean senescent CD8⁺ T-cell percentages over time. n values for part 1 baseline, Ext Scr, and years 1, 2, 3, 4, 5, and 6 are as follows: 6, 6, 4, 3, 3, 2, 1, and 3, respectively. (C) Mean CD4⁺ and CD8⁺ T-cell percentages overtime. n values for part 1 baseline, Ext Scr, and years 1, 2, 3, 4, 5, and 6 are as follows: 6, 6, 6, 5, 6, 5, 3, and 4, respectively. (D) Mean naïve T-cell percentages (CD4⁺ or CD8⁺ CD45RACD62L⁺) over time. n values for part 1 baseline, Ext Scr, and years 1, 2, 3, 4, 5, and 6 are as follows: 6, 6, 4, 4, 5, 2, 1, and 3, respectively. (E) Individual and mean naïve B-cell percentages (CD19⁺CD27⁻CD10⁻) over time. n values for DFT baseline, Ext Scr, and year 1 are as follows: 6, 5, 5. (F) Individual and mean switched memory B-cell percentages (CD19⁺CD27⁺IgD⁻) over time. n values for DFT baseline, Ext Scr, and year 1 are as follows: 6, 5, and 5, respectively. (G) Individual and mean nonswitched memory B-cell percentages (CD19⁺CD27⁺IgD⁺) over time. n values for DFT baseline, Ext Scr, and year 1 are as follows: 6, 4, and 5, respectively. (H) Individual and mean transitional B-cell (CD19⁺CD27⁻CD10⁺ or CD20⁺CD10⁺) percentages over time. n for DFT baseline, Ext Scr, and years 1, 2, 3, 4, 5, and 6 are as follows: 6, 6, 5, 4, 5, 3, 1, and 3, respectively. Normal ranges are from personal communication (panels A-B; Manish Butte, University of California, Los Angeles, email, 29 March 2022) and literature (panels C-H).^29,30 In panels A-B,E-H, gray boxes indicate normal ranges. In panels C-D, gray boxes indicate normal ranges for CD4⁺ cells, and dotted lines indicate normal ranges for CD8⁺ cells. Error bars are ± standard error of the mean. Ext Scr, extension screening.