Effects of VWF OLG truncation on Golgi morphology and WPB cargo storage. (A) Immunofluorescent images of VWF (green), TGN46 (red), and DAPI (blue) of BG-treated HUVECs (2 mM for 72 hours) compared with untreated CTRLs (representative images of 2 independent experiments). (B) Immunofluorescence images of VWF (green), Angpt-2 (red), and DAPI (blue) of BG-treated HUVECs compared with untreated CTRL cells (yellow arrowheads indicate WPBs positive for Angpt-2; representative images of n = 5). (C) Angpt-2 levels secreted into conditioned media from BG-treated HUVECs compared with untreated CTRLs (n = 5; t test; P = .0062).