BG treatment of HUVECs alters VWF O-linked glycosylation. (A) Inhibitory effect of BG on O-glycan biosynthetic processing; (B) VWF immunoblot in HUVEC cell lysates and conditioned media after BG treatment (GAPDH loading control; representative blot of n = 3). (C) M amurensis lectin II (MAL-II) binding to VWF secreted from BG-treated vs untreated HUVEC controls cells (CTRL) (mean and standard error of the mean [SEM]; n = 4; simple linear regression; P < .0001). (D) PNA lectin binding to VWF secreted from BG-treated vs untreated HUVEC CTRLs (mean and SEM; n = 4; simple linear regression; P = .0063). A.U., arbitrary units; proVWF, VWF with propeptide.