Platelet activation in Jak2^WT and Jak2^V617F mice. Activation of αIIbβ3 integrin using binding of (A) labeled fibrinogen and (B) platelet P-selectin expression are measured in platelets using flow cytometry in both Jak2^WT and Jak2^V617F mice at baseline and at day 1 after sham-surgery or CVST. (C) Bar graph indicating plasma levels of soluble P-selectin (sP-Sel). Results are expressed as mean ± standard deviation, n = 7 to 8 per group. Data were analyzed using Kruskal-Wallis test followed by post hoc Dunn test. ∗P < .05; ∗∗P < .01.