Clonal heterogeneity and inferred timing of genetic drivers. (A) Proportion in which recurrent drivers are found as clonal or subclonal across the 67 samples (top), along with the individual CCF values for each sample affected by a driver (bottom). Median CCF values are shown (bottom, bars represent the median and interquartile range for each driver). (B) Correlation matrix for the most recurrent genomic alterations for the 48 patients with MF, with Fisher exact test to detect such significant pair of mutations. (C) Timing of genomic alterations with early events at top and late events at bottom. Color indicates alteration types. Arrows between 2 alterations were drawn when 2 drivers were found in 1 sample with an excess of clonal to subclonal events. Dashed arrows indicate 1 clonal-subclonal pair and solid arrows indicate ≥2 clonal-subclonal pairs.