Effect of PK deficiency on extrinsic pathway–initiated TG. (A) Representative TF-initiated TG curves in Klkb1^+/+ and Klkb1^–/– mouse PPP. Quantification of TG lag time (B) and peak TG (C) in Klkb1^+/+ and Klkb1^–/– mouse PPP initiated with 0.05 pM and 0.5 pM TF (n = 5 per group). (D) Representative TF-initiated TG curves in Klkb1^+/+ and Klkb1^–/– mouse WB. Quantification of TG lag time (E) and peak TG (F) in Klkb1^+/+ and Klkb1^–/– mouse WB initiated with 0.05 pM and 0.5 pM TF (n = 6-8 per group). Data are presented as individual values with median and interquartile range. Data were analyzed with Mann-Whitney U tests comparing Klkb1^+/+ and Klkb1^–/– samples under the same trigger conditions; ∗∗P < .01.