Hepatic congestion is enhanced in SS^BM/R46Q mice. (A) Livers were collected and stained with H&E; representative images of livers from SS^BM/WT, SS^BM/R41Q, and SS^BM/R46Q mice during steady state (SAL) and TNF-α challenge; scale bar represents 50 μm. Three blinded observers scored congestion (B) and necrosis (C). Data represent mean ± SEM. Black arrow denotes sinusoidal congestion, and green arrow denotes iron-laden macrophages. There were 6 to 8 mice (SAL, steady state) and 15 to 17 mice (TNF-α challenge) per group. Asterisks above bar represent statistical significance vs SAL-treated mice of same genotype by 2-way ANOVA and Tukey post hoc test. Asterisks above brackets indicate comparison. ∗P < .05. SAL, saline.