Inhibition of endogenous APC by SPC54 exacerbates thromboinflammation in HbSS mice after TNF-α challenge. (A) Male and female HbSS mice were treated with IgG or SPC-54 (10 mg/kg, IP) 19 hours before SAL or TNF-α (2 μg/kg, IP) and plasma was collected 5 hours later. Plasma levels of TAT (B), IL-6 (C), IL-18 (D), sVCAM1 (E), sICAM (F), VWF (G), and sP-sel (H). Data represent mean ± SEM mean of 5 to 10 mice per group. Asterisks above brackets indicate statistical significance by 1-way ANOVA with Kruskal-Wallis posttest. ∗P < .05; ∗∗∗P < .001; ∗∗∗∗P < .0001. Sal, saline.