APC inhibition exacerbates hepatic congestion and lung neutrophil accumulation. (A) Representative images of liver sections from HbSS mice treated with IgG or SPC-54 stained with H&E (top) and Prussian Blue (bottom). Black arrow indicates sinusoidal congestion, and green arrow indicates iron-laden macrophages. Quantification of liver congestion (B) and liver necrosis (C) by 3 blinded observers. (D) Plasma levels of alanine aminotransferase (ALT). (E) Quantification of Prussian blue stain per total area. (F) Representative images of lung sections stained for neutrophils (brown). Scale bar, 50 μm; red asterisk (∗) denotes neutrophils. (G) Quantification of neutrophils (PMN) averaged over 10 high powered (40×) fields. Data are represented by mean ± SEM of 5 to 6 mice per group and analyzed by 2-way ANOVA and Tukey post hoc test. Asterisks directly above the bars indicate the statistical significance of SS mice to AA mice in the same treatment group. Asterisks over brackets indicate difference from IgG-treated mice. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; and ∗∗∗∗P < .0001. AA, controls; PMN, polymorphonuclear lymphocytes; SS, sickle mice.