Inhibition of endogenous APC by SPC-54 exacerbates the thromboinflammation at steady state in HbSS mice. (A) Four-month-old HbAA (gray) and HbSS (blue) mice were treated with 10 mg/kg (i.p.) IgG (solid bars), or SPC54 (white hashed bars), and samples were collected 24 hours later. Plasma levels of TAT (B), IL-6 (C), IL-18 (D), sVCAM (E), sICAM (F), VWF (G), sP-sel (H), and PF4 (I). Data are represented by mean ± standard error of the mean (SEM) of 5 to 6 mice per group and analyzed by 2-way analysis of variance (ANOVA) and Tukey post hoc test. Asterisks directly above the bars indicate statistical significance of SS mice to AA mice in the same treatment group. Asterisks over brackets indicate difference from IgG-treated mice. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; and ∗∗∗∗P < .0001. AA, controls; i.p., intraperitoneal; SS, sickle mice.