Gene expression profile of the MDS clone with high HMGA2 expression. (A) Principal component analysis (PCA) of RNA-seq data of c-Kit⁺ BM HSC/Ps obtained from indicated mice. (B) Hierarchical Ordered Partitioning and Collapsing Hybrid (HOPACH)-generated clusters of differentially expressed genes (mean values of each group were used) with statistically enriched Gene Ontology (GO) terms (left) and enriched GO term–associated genes (right) in c-Kit⁺ BM HSC/Ps obtained from indicated mice. (C-E) GSEA plots showing the ranked genes in RUNX1^S291fs/HMGA2 c-Kit⁺ BM cells relative to cells from Empty mice. (F) PCA of RNA-seq data of Ly6G⁺ BM granulocytes obtained from indicated mice. (G) HOPACH-generated clusters of differentially expressed genes with statistically enriched GO terms (left) in granulocytes obtained from indicated mice. (H) Genes upregulated in both RUNX1^S291fs and RUNX1^S291fs/HMGA2 granulocytes relative to cells from Empty mice. (I) GO term enrichment analysis of 120 genes indicated in panel H. The top 10 GO terms for cellular component, biological process, and molecular function categories are shown. (J) Genes upregulated in RUNX1^S291fs/HMGA2 granulocytes relative to granulocytes from Empty and RUNX1^S291fs mice. (K) GO term enrichment analysis of 402 genes indicated in panel J. Top 10 GO terms for cellular component, biological process, and molecular function categories are shown.