HMGA2 overexpression in HSC/Ps results in impaired granulopoiesis and neutropenia in MDS mice. (A) Schematic of experimental design. (B) Survival of Empty (n = 5), RUNX1^S291fs (n = 5), HMGA2 (n = 6), and RUNX1^S291fs/HMGA2 mice (n = 6). (C) White blood cell (WBC) counts, hemoglobin (Hb) concentration, and platelet counts in the PB of mice. Data are represented by the mean ± standard deviation (SD). (D-E) Flow cytometric analysis of BM cells of indicated mice (n = 5 per each group). Frequency of c-Kit⁺ fraction is shown in panel E. Data are presented as the mean ± SD. (F-G) Flow cytometric analysis of BM cells of indicated mice (n = 5 per each group). Frequency of granulocyte fraction is shown in panel G. Data are presented as the mean ± SD. (H-J) Flow cytometric analysis of BM cells of indicated mice (n = 5 per each group). Frequency of CD150⁺ CD48⁻ HSC, CD150⁻ CD48⁻ MPP, CD150⁻ CD48⁺ HPC1, and CD150⁺ CD48⁺ HPC2 in BM cells is shown in panel I. Data are presented as the mean ± SD. Frequency of LSK, lineage marker–negative c-Kit⁺ (LK), CMP, GMP, and in BM cells is shown in panel J. Data are presented as the mean ± SD. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001. CMP, common myeloid progenitors; GMP, granulocyte-macrophage progenitors; LSK, lineage marker− Sca1+ c-Kit+ cells; MEP, megakaryocyte-erythroid progenitors; MPP, multipotent progenitors.