Interaction of PSGL-1 and P-selectin regulates adhesion-related signaling and β-integrin activation in MM cells. (C) HUVECs were treated with or without GMI-1070 (250 or 500 μM for 1 hour), nontreated MM1s cells were cocultured with the HUVECs for 1 hour, and MM1s cells not cocultured with HUVECs served as a control. MM cells were then separated from the HUVECs, lysed, and whole-cell lysates were subjected to Western blotting for pFAK, pAKT, pCoffilin, pSRC, and p-GSK3α/β. Coculture of MM cells with HUVECs induced adhesion-related signaling in MM cells that was reversed by GMI-1070.