Transplantations of BM cells from primary recipients into secondary recipients. (A) Schematic drawing of the experimental setup for noncompetitive BM transplantations into secondary recipient mice (n = 6 mice per group). (B) Percentages of mice that showed engraftment defined as >1% GFP chimerism in Gr1⁺ cells in peripheral blood at 20 weeks after transplantation. (C) Spleen weight at terminal workup 20 weeks after transplantation. (D) Time course of blood counts and GFP chimerism in secondary recipients. (E) GFP chimerism in LT-HSCs at terminal workup 20 weeks after transplantation. (F) Schematic drawing of the experimental setup for competitive BM transplantations into secondary recipients at 1:50 dilution (n = 12 mice per group). (G-J) Same annotations as in panels B-E. ANOVA with subsequent Tukey (D,I) posttest or 1-way ANOVA with subsequent Tukey (E, H) posttest were used. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.