Endothelial Slc39a8-deficient mice have altered liver Bmp6 expression when fed a high-iron diet. Liver endothelial cells (LECs) were isolated from Slc39a8^fl/fl;Stab2-Cre⁺ and Cre^– mice by magnetic separation (n = 5-6/group) and analyzed for enrichment by expression of endothelial-specific marker (A) Cd31 compared with whole liver, and expression of (B-C) Slc39a8 relative to Rpl19 by qRT-PCR in whole liver and endothelial cells, respectively. In (A), cells from Cre⁺ and Cre^– mice were combined for enrichment analysis for a total of n = 10 to 11 per group. (D-G) Five-week-old male (n = 5-8/group) and female (n = 6-8/group) Slc39a8^fl/fl;Stab2-Cre⁺ and littermate Cre^– mice were fed standard rodent chow (380 ppm iron) or purified high-iron diet (10 000 ppm iron) for 3 weeks and analyzed at 8 weeks for (D) hepatic expression of Bmp6 and (E) Hamp relative to Rpl19 by qRT-PCR, (F) liver iron, and (G) serum iron concentration. Bar graphs represent mean ± SEM, with individual points indicating the number of animals. Statistical differences were determined by (A-C) the 2-tailed Student t test for normally distributed values or (D-G) the 2-way analysis of variance followed by the 2-stage step-up method of the Benjamini, Krieger, and Yekutieli test to correct for multiple comparisons by controlling the false discovery rate (Q = 0.05). ns, not significant.