Prmt1 KO replicates the transcriptomic alterations induced by dKO. (A) Overlap of DEGs from Prmt1 KO and dKO LSKs. (B) Gene set enrichment analysis of Prmt1 KO LSKs. Heatmap illustrating the log₂fold suppression of DNA replication genes (C) and DNA repair genes (D) in single and dKO LSKs compared with WT LSKs. Prmt1^fl/fl and β-catenin^fl/fl mice treated with corn oil (vehicle) served as WT control for Prmt1 KO and Hoxa9/β-catenin KO, respectively. Asterisks (∗) indicate significant change in gene expression (P adjusted ≤ .05). (E) Profile plots of ATAC-seq data (RPKM-normalized) around peak center of DNA replication and repair genes. (F) Chromatin accessibility at Mcm5, Mcm2, and Pold3 loci. WT control represents BL6 mice treated with corn oil (vehicle).