Impaired quiescence and DNA replication dynamics induced by Prmt1 KO. (A) Representative fluorescence-activated cell sorting plots of Ki67 and Hoechst 33342 staining of LSKs isolated from indicated mice 2 weeks after tamoxifen treatment. (B) Percentage of LSKs in G0 cell cycle. Percentage Ki67+ (C) and BrdU+ (D) LSKs from indicated mice (n = 3 per group). (E) CldU track length of DNA fibers from HSPCs isolated from indicated mice. (F) Representative images of elongating fork lengths. Scale bar, 10 μm. (G) Ratio of IdU (red) track lengths of bidirectional forks. (H) Percentage of stalled forks (green only). Fiber data from 1 representative experiment are shown (n = 3, data are shown in supplemental Figure 4). Representative immunofluorescent images (I) and ƳH2AX focus counts (J) of ckit+ Prmt1 WT and KO cells. Several fields of cells were scored from 2 independent experiments. Scale bars, 25 μm. WT BL6 mice treated with corn oil (vehicle) are indicated as WT control. ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001.
