FOXK2 cooperates with RUNX1R320∗ to regulate MYC. (A) Analysis of K562 H3K27ac (ENCODE ENCFF465GBD), FOXK2 (ENCODE ENCFF286IOU), RUNX1 wild-type, and RUNX1R320∗ binding at the MYC and MYC enhancer regions upstream and downstream of MYC (top). GRID-seq long-range interaction map of chromatin-associated RNAs at MYC locus (bottom). Interaction strength with a greater score between RUNX1 and RUNX1R320∗ is denoted in blue and red, respectively. (B-C) CUT&RUN qPCR analysis of FOXK2, RUNX1, and RUNX1R320∗ at MYC BENC enhancer element 3 ‘E3’. (D-E) Western blots examining FOXK2 and MYC protein levels in wild-type (WT) RUNX1 and RUNX1R320∗ cells transduced with nontargeting shCtl or FOXK2 shRNAs with a β-actin loading control. (F) Model describing the roles of RUNX1R320∗ and FOXK2 in MYC enhancer regulation. Student t test was used to determine significance: ∗ P ≤ .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.
