Proteomic landscape of platelets from controls and patients before and after treatment. Volcano plot comparing proteomic profiles before and after treatment of individual patients. (A) Index case A-II-1. (B) Index case A-II-2. (C) Index case A-II-3. (D) Index case B-II-1. Upregulated proteins in patients are shown in orange, whereas downregulated proteins are shown in dark blue. Difference in expression is shown on the x-axis, and the logarithmic P value (–log(P value)) is shown on the y-axis. Dotted lines indicate the threshold of significance with the horizontal line marking a –log10 (adjusted P value) of 1, and the vertical dotted lines marking a log2(fold change) of 1. (E) GO term enrichment of functional modules. Color indicates module, node size indicates amount of proteins with a GO term annotation, and edge width denotes GO term similarities based on Jaccard’s similarity. (F) Box plots showing the protein intensities (LFQ) of a selection of mitochondrial proteins. Black dots represent individual measurements.