QCG preincubation reduces oxidative impact of H₂O₂ induced stress. (A) Fold change in intracellular ROS detected by DCFDA staining and flow cytometry in RBCs (n = 6). (B) Representative ektacytometry curve as cells pass through osmotic gradient at a constant shear stress of 16 Pa. (C) Maximum deformability and (D) hydration level of RBCs from ektacytometry curves (n = 3). Mean ± standard error of the mean denoted by error bars. A 1-way paired t test was used to analyze the data. ns, not significant; ∗P < .05; ∗∗P < .005; ∗∗∗P < .0005.