CD45-ADC (1.5 or 3 mg/kg) conditioning facilitates alloengraftment in an MHC–mismatched transplant model. (A-B) Female or male Fancc^−/− (B6, H-2^b) mice (10- to 16-weeks-old) were conditioned with 3 mg/kg isotype-ADC (n = 7) or CD45-ADC (n = 14) on d2 and received 40 × 10⁶ BALB/c (H-2^d) donor BM cells on d0. ADC–conditioned Fancc^−/− recipients were also treated with anti-CD40L mAb (200 μg) from d1 through d+5, then 2× per week until d+14 post-BMT. (A) Engraftment of donor cells (H-2^d) in PB of mice who underwent transplant were analyzed at 5, 10, and 20 weeks post-BMT by flow cytometry, and PB multilineage donor chimerism was analyzed at 20 weeks post-BMT (B). Data represent mean ± SEM. ∗∗P < .01 and ∗∗∗∗P < .0001 using the 2-tailed Student t test. Pooled data from 2 experiments are shown. (C-G) Female or male Fancc^−/− mice (10- to 16-weeks-old) were conditioned with isotype-ADC (1.5 mg/kg) or CD45-ADC (1.5 or 3 mg/kg) on d2 and then received 40 × 10⁶ donor (H-2^d) BM cells on d0. CD45-ADC–conditioned recipients were also treated with anti-CD40L mAb as mentioned above. (C) Engraftment of donor cells (H-2^d) in PB of mice who underwent transplant were analyzed at 5, 20, and 33 weeks post-BMT, and PB multilineage donor chimerism was analyzed at 33 weeks post-BMT (D). (E-G) Recipients were killed at 34 weeks post-BMT and donor (H-2^d) chimerism in BM (E), thymus (F), and spleen (G) were analyzed. For panels C-G, data represent mean ± SEM (n = 4-7 mice per group). ∗P < .05, ∗∗∗P < .001, and ∗∗∗∗P < .0001 using the 2-tailed Student t test.