TNF signaling components are induced in vivo in response to anti-CD3 in T-ALL. RNA obtained from leukemic cells (T-ALL case UPNT776) of engrafted mice that were either left untreated, or treated for the indicated time periods (hours) with anti-CD3 OKT3 were analyzed using a Nanostring custom panel of 97 genes comprising 5 housekeeping genes. Histograms show digitalized messenger RNA expression for TNF⍺, LT⍺, BIRC3, NF-κB1, NF-κB2, c-REL, RELB, TRAF1, TRAF3, NF-κBIA, NF-κBID, and NF-κBIE after normalization with Nsolver software. Glucuronidase beta (GUSB), glucose-6-phosphate dehydrogenase (G6PD), and RNA polymerase 2 subunit A (POLR2A) were used as control housekeeping genes.