Therapeutic plasminogen activation accelerates cVWF formation. Levels of VWF antigen (A), cVWF (B), and fraction of circulating VWF that exists in a plasmin-cleaved state (C) after Microlyse treatment (blue squares) or saline treatment (red circles) in Adamts13^−/− mice, challenged by administration of rhVWF. Data are displayed as scatter plots with medians, and represent 3 independently executed experiments. Results were analyzed by 2-way analysis of variance followed by the Šidák’s post-hoc test. ∗∗P < .005; ∗∗∗P < .005; ∗∗∗∗P < .0001; ns, nonsignificant.