Characterization of a binding site in cVWF that enables V_HH capture. Conditioned cell culture supernatants containing C-terminally truncated VWF variants were incubated with plasmin (150 μg/mL) or vehicle and analyzed by (A) western blotting and by ELISA. (B) V_HH G5 was immobilized, and captured VWF variants were detected with a polyclonal antibody. (C) Western blot of captured VWF products, spiked in buffer or NPP. Data represent 3 independently executed experiments. Bar graphs show means ± SD. M, marker; Neg. Sup, negative control supernatant; Plm, plasmin.