Time-dependent VWF cleavage by plasmin during microthrombus breakdown. (A) Time course of sampling during microthrombus breakdown by plasmin (75 μg/mL). Plasmin activity was blocked with PPACK, cells removed by centrifugation, and supernatants analyzed by western blotting. (B) Western blot under nonreducing conditions; (C) western blot under reducing conditions. Data represent 3 independently executed experiments. Plm, plasmin.