Analysis of metabolic parameters of mice from the experiment shown inFigure 3 (n = 12 per group). (A) Left panel: Time course of nonfasting blood glucose levels. Right panel: glucose tolerance test. Blood glucose concentration was measured at different time intervals after injecting glucose IP in all the mice treated in the experiment, at week 6 of treatment. In light purple, untreated WT mice (n = 6) analyzed separately. (B) Glutamine (Gln) concentration in the serum from treated mice at the end of the experiment (week 6) and from untreated WT mice (n = 3). C) Left panel: measurement of ECAR upon injection of glucose, oligomycin, and 2-DG, as an indication of lactate production. Right panel: OCR upon administration of oligomycin, FCCP, and Antimycin A, indicative of mitochondrial oxidative phosphorylation. Both experiments were performed in a 96-well plate Seahorse assay, with 2 × 105 BM cells per well (n = 12 mice per group). In light purple, results from BM cells from untreated WT mice (n = 6). The plots represent the mean ± SEM. 2-way ANOVA followed by Dunnett’s test for multiple comparison was applied in panels A,C and 1-way ANOVA with Dunnett’s test in panel B. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001.
