CD33/CD123 NANOBODY TCE kills monocytes and induces cytokine production in an autologous PBMC setup. Flow cytometry plot depicts the expression of CD33 and CD123 within human PBMC (A). Dose dependent monocyte depletion in PBMC by tested mono- and bispecific TCE is shown as CD14% determined in flow cytometry (B, 1 representative donor out of 8 is shown). The global geometric EC₅₀ (half-effective dose) derived from the dose response curves of 8 different donors is shown to the right of the graph. Dose dependent cytokine release within human PBMC is shown for the same representative donor (C).