CyTOF immunophenotypic profiling revealing subsets of AML and T cells responding to CD33/CD123 NANOBODY TCE. CyTOF immunophenotyping was performed on primary AML PBMC samples. Uniform manifold approximation and projection (UMAP) plots displaying the expression (left) and clustering (right) of CD34+ (A) and CD3+ (B) in 3 samples examined. Bar graphs in panels A and B displaying the frequency of each cluster (subset) at the indicated treatment in 3 samples. Heat maps show the expression level of surface markers in each subset. Subsets and markers of interest are highlighted with rectangle boxes.