Characteristics of somatic mutations between HCMBL and CLL. (A) Heat map representing the distribution of high-impact and hot spot mutations in 59 genes frequently mutated in CLL and HCMBL. Each column represents a single individual from our cohort. The external bar indicates individuals with HCMBL (gray) or CLL (red) diagnosis; the second bar stratifies the heat map by CLL-IPI (low risk [green], intermediate risk [yellow], and high/very high risk [red]); the third bar represents the TML distribution (ie, the number of mutated genes per individual); then the next 5 bars represent clinical components: Rai stage (0-gray; I-green; II-yellow; III-red; and IV-purple), sex, IgHV, B2M, and del(17p) by fluorescence in situ hybridization. Finally, the internal bars represent the list of mutated genes in our study color-coded by the type of mutation: missense (yellow), in frame (gray), frameshift (red), nonsense (purple), splice (blue), and >1 mutation (green). (B) Distribution of the variant allele fraction of high-impact and hot spot mutations between individuals with CLL and HCMBL. (C) Distribution of mutation type between individuals with HCMBL and CLL. B2M, Beta-2 microglobulin; FISH17p, fluorescence in situ hybridization 17p deletion.