American Society of Hematology

$Modeling the in vivo trafficking of human HSPCs through IS clonal tracking. Venn diagrams show a schematic representation of the groups considered for the IS sharing analysis in each figure and report the IS number of the compared groups. (A) Histogram showing the relative frequencies of IS shared between BM PRIMITIVE (HSC + MPP, red), LYMPHOID (MLP + PreBNK, green), and MYELOID (CMP + GMP + MEP, blue) HSPC subsets and PB-HSPCs. (B) Histogram showing the relative frequencies of IS shared (yellow) and not shared (purple) between BM-HSPCs from 2 BM distant sites also recaptured in PB-HSPCs. (C) Histogram showing the relative frequencies of IS shared between BM PRIMITIVE (red), LYMPHOID (green), and MYELOID (blue) HSPC subsets from 2 BM distant sites also recaptured in PB-HSPCs. (D) Histogram showing the relative frequencies of IS shared between BM- or PB-HSPCs and PB T cells. (E) Histogram showing the relative frequencies of IS found in the shared fraction between BM-MLP and PB-HSPC (yellow), only in PB-HSPCs (blue) or only in BM-MLPs (red) that are also shared (dark colors) or not shared (light colors) with PB T cells. (F) Histogram showing the relative frequencies of IS found only in BM-HSPCs (red), only in PB-HSPCs (blue), and in the shared fraction between BM- and PB-HSPCs (yellow) that are also shared (dark colors) or not shared (light colors) with PB mature lineages. (G) Histograms showing the relative frequencies of IS found only in BM-HSPCs (red), only in PB-HSPCs (blue), and in the shared fraction between BM- and PB-HSPCs (yellow) that were detected in both myeloid and lymphoid (multilineage), in only myeloid (only myelo) and in only lymphoid (only lympho) PB mature cells. (H) Density plots representing the log10 sequence count distribution relative to IS found in BM-HSPCs (left) and in PB-HSPCs (right). The dot line refers to the clones shared between the 2 sources, whereas solid lines show the clonal abundance of not shared IS. Mann-Whitney test was performed to compare the sequence count distribution. For panels A-E,G, the P values shown in the figure were computed through Fisher exact test. Whenever >2 groups were compared, the nominal P values were adjusted with Bonferroni correction. Only significant P < .05 are shown in the plots.$

Modeling the in vivo trafficking of human HSPCs through IS clonal tracking. Venn diagrams show a schematic representation of the groups considered for the IS sharing analysis in each figure and report the IS number of the compared groups. (A) Histogram showing the relative frequencies of IS shared between BM PRIMITIVE (HSC + MPP, red), LYMPHOID (MLP + PreBNK, green), and MYELOID (CMP + GMP + MEP, blue) HSPC subsets and PB-HSPCs. (B) Histogram showing the relative frequencies of IS shared (yellow) and not shared (purple) between BM-HSPCs from 2 BM distant sites also recaptured in PB-HSPCs. (C) Histogram showing the relative frequencies of IS shared between BM PRIMITIVE (red), LYMPHOID (green), and MYELOID (blue) HSPC subsets from 2 BM distant sites also recaptured in PB-HSPCs. (D) Histogram showing the relative frequencies of IS shared between BM- or PB-HSPCs and PB T cells. (E) Histogram showing the relative frequencies of IS found in the shared fraction between BM-MLP and PB-HSPC (yellow), only in PB-HSPCs (blue) or only in BM-MLPs (red) that are also shared (dark colors) or not shared (light colors) with PB T cells. (F) Histogram showing the relative frequencies of IS found only in BM-HSPCs (red), only in PB-HSPCs (blue), and in the shared fraction between BM- and PB-HSPCs (yellow) that are also shared (dark colors) or not shared (light colors) with PB mature lineages. (G) Histograms showing the relative frequencies of IS found only in BM-HSPCs (red), only in PB-HSPCs (blue), and in the shared fraction between BM- and PB-HSPCs (yellow) that were detected in both myeloid and lymphoid (multilineage), in only myeloid (only myelo) and in only lymphoid (only lympho) PB mature cells. (H) Density plots representing the log10 sequence count distribution relative to IS found in BM-HSPCs (left) and in PB-HSPCs (right). The dot line refers to the clones shared between the 2 sources, whereas solid lines show the clonal abundance of not shared IS. Mann-Whitney test was performed to compare the sequence count distribution. For panels A-E,G, the P values shown in the figure were computed through Fisher exact test. Whenever >2 groups were compared, the nominal P values were adjusted with Bonferroni correction. Only significant P < .05 are shown in the plots.

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