cHSPCs display reduced expression of molecules controlling BM retention. (A) Heat map showing the average normalized gene expression of factors controlling BM retention (top) and the percentage of cells coexpressing genes encoding for integrins driving BM homing (dimer-DP cells) (bottom) in all cells as well as in cells from cluster 1 (LT-HSC) of BM (red) and PB (blue) origin. Asterisks show statistically significant differential frequencies of DP cells between the 2 sources (χ2 test, Bonferroni-adjusted P < .05). (B) Bar plots showing the average normalized expression of genes encoding for single integrin chains in ITGA4:ITGB1-, ITGA6:ITGB1-, and ITGA9:ITGB1-DP cells from total data set and LT-HSC (cluster 1) of BM (red) and PB (blue) origin. Asterisks show statistically significant differential gene expression between the 2 sources (2-sided Student t test, Bonferroni-adjusted P < .05). (C) Violin plots showing CXCR4 mean intensity of fluorescence (MFI) in total HSPCs, HSCs, and MPPs derived from HD-BM (n = 30) and -PB (n = 26). Mann-Whitney statistical test was applied for groups’ comparison and single P values are reported within the graph. AvgBM, average normalized gene expression in BM cells; AvgPB, average normalized gene expression in PB cells.
