Single-cell transcriptional and phenotypic profiling of human PB– and BM–derived HSPC subpopulations. (A) UMAP embedding, coloring cells by BM (red) and PB (blue) sources. (B) UMAP embedding showing 18 seurat clusters identified after unsupervised clustering. (C) UMAP embedding showing seurat clusters 1 to 17 and subclusters of seurat cluster 0. The legend on the right shows the annotation of the single clusters. (D) Stacked bar graph showing the distribution of the transcriptional clusters in BM- and PB-HSPCs. The dominant transcriptional signatures identified for PB-HSPCs are reported. (E) UMAP embedding showing the distinct phenotypic HSPC subpopulations identified by antibody derived tag (ADT) protein barcoding. (F) Distribution of the transcriptional clusters in the 10 ADT–defined HSPC subpopulations. Clusters are grouped according to the expressed HSPC transcriptional signature.