Figure 1.
cHSPC count and composition change in association with aging and hematopoietic impairment. (A) Correlation between cHSPC absolute count and age of healthy donors (HDs) analyzed. (B-C) Stacked bar graphs displaying the absolute counts (B) and relative composition (C) of PB-HSPC subsets in HDs of distinct age classes. HDs were classified according to age into neonates (NEON), infants (with 3 subgroups: PED 1, PED 2, PED 3), adolescent (PED 4), adult (AD), and older (AGED), as reported in supplemental Table 1. (D) Cell count of cHSPC in PED1/2 group, PED3/4 group, and patients affected by WAS (n = 12), ADA (n = 11), THAL (n = 7), and BMFS (n = 5). Pediatric individuals are represented as triangle (PED1), circles (PED2), square (PED3), and diamonds (PED4) according to their age. Mann-Whitney statistical test was applied for groups’ comparison, and single P values are reported within the graph. (E) Stacked bar graphs displaying the relative composition of cHSPC subsets in PED1/2 groups, PED3/4 groups, and patients with WAS, ADA and THAL. Results of Mann-Whitney statistical test applied for groups’ comparison are reported in supplemental Table 8. (F) Correlation between the number of colony-forming units (CFUs) obtained from 250 μL of whole PB and the age of healthy donors. (G) Correlation between the CFUs retrieved from 250 μL of whole PB and the absolute counts of cHSPCs in patients with WAS, ADA-SCID, THAL, and BMFS. (H) Stacked bar graph displaying the compositions of BM- and PB-HPSCs derived from age-matched healthy individuals (PED and AD). (I) Circulation indexes (CIs) of the single HSPC subpopulations in pediatric and adult donors. Mann-Whitney statistical test was applied for pediatric vs adult CI comparisons, and single P values are reported within the graphs. Kruskal-Wallis test with Dunn multiple comparisons was applied for CI comparisons among the distinct HSPC subsets, and the results are reported in supplemental Table 9. (B-C,E,H) Data are shown as mean with standard error of the mean (SEM). (I) Each dot represents a single individual, and the colored bars show the median value. (A,F-G) Statistical test for correlation: Spearman r. Spearman correlation coefficient (r) and P values are reported in each figure.

cHSPC count and composition change in association with aging and hematopoietic impairment. (A) Correlation between cHSPC absolute count and age of healthy donors (HDs) analyzed. (B-C) Stacked bar graphs displaying the absolute counts (B) and relative composition (C) of PB-HSPC subsets in HDs of distinct age classes. HDs were classified according to age into neonates (NEON), infants (with 3 subgroups: PED 1, PED 2, PED 3), adolescent (PED 4), adult (AD), and older (AGED), as reported in supplemental Table 1. (D) Cell count of cHSPC in PED1/2 group, PED3/4 group, and patients affected by WAS (n = 12), ADA (n = 11), THAL (n = 7), and BMFS (n = 5). Pediatric individuals are represented as triangle (PED1), circles (PED2), square (PED3), and diamonds (PED4) according to their age. Mann-Whitney statistical test was applied for groups’ comparison, and single P values are reported within the graph. (E) Stacked bar graphs displaying the relative composition of cHSPC subsets in PED1/2 groups, PED3/4 groups, and patients with WAS, ADA and THAL. Results of Mann-Whitney statistical test applied for groups’ comparison are reported in supplemental Table 8. (F) Correlation between the number of colony-forming units (CFUs) obtained from 250 μL of whole PB and the age of healthy donors. (G) Correlation between the CFUs retrieved from 250 μL of whole PB and the absolute counts of cHSPCs in patients with WAS, ADA-SCID, THAL, and BMFS. (H) Stacked bar graph displaying the compositions of BM- and PB-HPSCs derived from age-matched healthy individuals (PED and AD). (I) Circulation indexes (CIs) of the single HSPC subpopulations in pediatric and adult donors. Mann-Whitney statistical test was applied for pediatric vs adult CI comparisons, and single P values are reported within the graphs. Kruskal-Wallis test with Dunn multiple comparisons was applied for CI comparisons among the distinct HSPC subsets, and the results are reported in supplemental Table 9. (B-C,E,H) Data are shown as mean with standard error of the mean (SEM). (I) Each dot represents a single individual, and the colored bars show the median value. (A,F-G) Statistical test for correlation: Spearman r. Spearman correlation coefficient (r) and P values are reported in each figure.

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