Candidate genes identified by GWAS regulate FVIII release from HLECs. HLECs were transfected with small interfering RNA (siRNA), including control siRNA (Scramble Ct), siRNA directed against CD36 (siCD36), siRNA directed against B3GNT2 (siB3GNT2), and siRNA directed against PDIA3 (siPDIA3). The cells were washed and fed with fresh media, cultured for 24 hours, and the media was collected. FVIII concentrations in the cell culture media were measured by enzyme-link immunosorbent assay (n = 6 ± SD; ∗P < .05 compared with Scramble Ct). SD, standard deviation.