Levels of serum antibodies in response to immunization with both T-cell–dependent and –independent antigens. (A-B) Serum IgM, IgG, IgG1, IgG2b, and IgG3 anti-NP levels before and after NP-Ficoll or NP-CGG immunization. (A) Mice were injected intravenously with 50 μg NP-Ficoll in PBS and 7 days postimmunization blood was collected for the analysis. (B) Mice were intraperitoneally immunized with 100 μg NP-CGG, 100 μL inject alum and 3 weeks after mice were intravenously boosted with 50 μg NP-CGG. Seven days after first and boost immunization, blood was collected for quantification of serum antibodies levels. ∗P < .05 and ∗∗P < .005 (Kruskal-Wallis test). All significant values were corrected by multiple comparison Dunn test (alpha: 0.05).