Figure 2.
Schematic representation of the 17C02-based molecules. (A) The gene that encodes for the 17C02-scFv with a linker (connecting the VH with the VL chains) as well as an additional RGGGGSGGGGS were used to connect the scFv to the N-terminal sequence of human albumin and a 6xHis tag at the C-terminal end of albumin. (B) The genes encoding the VL and VH of 17C02 were used to express a human IgG1 1-armed antibody with the LALA mutation to impair Fc-FcγR interactions, using the “knob-into-hole” strategy. (C) The genes that encode for the VL and VH of 17C02 were expressed as a full-length mouse IgG2a antibody.

Schematic representation of the 17C02-based molecules. (A) The gene that encodes for the 17C02-scFv with a linker (connecting the VH with the VL chains) as well as an additional RGGGGSGGGGS were used to connect the scFv to the N-terminal sequence of human albumin and a 6xHis tag at the C-terminal end of albumin. (B) The genes encoding the VL and VH of 17C02 were used to express a human IgG1 1-armed antibody with the LALA mutation to impair Fc-FcγR interactions, using the “knob-into-hole” strategy. (C) The genes that encode for the VL and VH of 17C02 were expressed as a full-length mouse IgG2a antibody.

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