Mobilized grafts from patients with myeloma contain high numbers of effector/effector memory CD8 T cells and Tregs. BM and PBSC were collected from the same patients with myeloma (n = 14-18). We performed FACS analysis to determine the phenotype of T cells in the BM and PBSC. Data from the BM and PBSC were combined and unbiased clustering was performed to visualize each population using FlowSOM. A heatmap was generated based on the expression levels of the flow cytometry markers. (A) Representative FACS plots and frequency of naïve and memory subsets in CD8 T cells. (B) Heatmap of marker expression and the frequency of each CD8 T-cell population. (C) Representative FACS plots and frequency of naïve and memory subset of conventional CD4 T cells. (D) Heatmap of marker expression and the frequency of each CD4 T-cell population. (E) Representative FACS plots of CD25⁺CD127⁻CD4 Tregs and the proportion of TIGIT⁺ Treg and TIGIT⁺CD39⁺ Treg. Each red dot and blue square represent a single BM and PBSC sample, respectively. Paired BM and PBSC are connected by solid line. (F) Frequency of TIGIT⁺ Treg and TIGIT⁺CD39⁺ Treg in PBSC from healthy donors (HD) and patients with myeloma (Pts). Wilcoxon matched pairs signed rank test for paired comparison and Welch test for 2 samples comparison (n = 18 per group). (G) Months to progressive myeloma after stem cell transplantation in cytogenetic high-risk patients with at least 12 months of follow-up relative to Treg within the stem cell grafts. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001.