Figure 2.
APC-released EVs are enriched with anti-inflammatory miRs. EVs isolated from HUVECs after treatment with a control vehicle or APC (100 nM) for 8 hours were quantified by NTA, and an equal number of EVs (1 × 109) were subjected to miR analysis by deep sequencing (UT Southwestern Core). (A) From the sequencing data, the top 25 most abundant and differentially expressed miRs are shown in the heat map. (B) Group comparison of scaled expression data set using nonparametric test visualized by volcano plot showing differentially expressed miRs between the APC-released EVs and control EVs with an adjusted P value < .05 and fold change of >2.0. (C) Validation of differentially expressed miRs between control EVs and APC-released EVs identified by deep sequencing. EVs isolated from HUVECs after challenging with a control vehicle or APC were subjected to miR isolation by mirVana miRNA isolation kit. The relative abundance of hsa-miR-423-5p, hsa-miR-92b-3p, hsa-miR-181b-5p, hsa-miR-30c-5p, and hsa-miR-362-5p were validated by quantitative real-time polymerase chain reaction. (D-F) GO enrichment analysis of inflammation-related target genes of differentially upregulated miRs such as hsa-miR-181b-5p (D), hsa-miR-28-3p (E), and hsa-miR-29b-1-5p (F) influencing various proinflammatory pathways (denoted in figures as “GO” identification number) (GO:1900227, Positive Regulation of NLRP3 Inflammasome Complex Assembly; GO:1900225, Regulation of NLRP3 Inflammasome Complex Assembly; GO:0150078, Positive Regulation of Neuroinflammatory Response; GO:0002675, Positive Regulation of Acute Inflammatory Response; GO:0090594, Inflammatory Response to Wounding; GO:0002526, Acute Inflammatory Response; GO:0050729, Positive Regulation of Inflammatory Response; GO:1900015, Regulation of Cytokine Production Involved in Inflammatory Response; GO:0006954, Inflammatory Response; GO:1900017, Positive Regulation of Cytokine Production Involved in Inflammatory Response; GO:0150077, Regulation of Neuroinflammatory Response; GO:0050727, Regulation of Inflammatory Response; GO:0106014, Regulation of Inflammatory Response to Wounding; and GO:0002861, Regulation of Inflammatory Response to Antigenic Stimulus). ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001.

APC-released EVs are enriched with anti-inflammatory miRs. EVs isolated from HUVECs after treatment with a control vehicle or APC (100 nM) for 8 hours were quantified by NTA, and an equal number of EVs (1 × 109) were subjected to miR analysis by deep sequencing (UT Southwestern Core). (A) From the sequencing data, the top 25 most abundant and differentially expressed miRs are shown in the heat map. (B) Group comparison of scaled expression data set using nonparametric test visualized by volcano plot showing differentially expressed miRs between the APC-released EVs and control EVs with an adjusted P value < .05 and fold change of >2.0. (C) Validation of differentially expressed miRs between control EVs and APC-released EVs identified by deep sequencing. EVs isolated from HUVECs after challenging with a control vehicle or APC were subjected to miR isolation by mirVana miRNA isolation kit. The relative abundance of hsa-miR-423-5p, hsa-miR-92b-3p, hsa-miR-181b-5p, hsa-miR-30c-5p, and hsa-miR-362-5p were validated by quantitative real-time polymerase chain reaction. (D-F) GO enrichment analysis of inflammation-related target genes of differentially upregulated miRs such as hsa-miR-181b-5p (D), hsa-miR-28-3p (E), and hsa-miR-29b-1-5p (F) influencing various proinflammatory pathways (denoted in figures as “GO” identification number) (GO:1900227, Positive Regulation of NLRP3 Inflammasome Complex Assembly; GO:1900225, Regulation of NLRP3 Inflammasome Complex Assembly; GO:0150078, Positive Regulation of Neuroinflammatory Response; GO:0002675, Positive Regulation of Acute Inflammatory Response; GO:0090594, Inflammatory Response to Wounding; GO:0002526, Acute Inflammatory Response; GO:0050729, Positive Regulation of Inflammatory Response; GO:1900015, Regulation of Cytokine Production Involved in Inflammatory Response; GO:0006954, Inflammatory Response; GO:1900017, Positive Regulation of Cytokine Production Involved in Inflammatory Response; GO:0150077, Regulation of Neuroinflammatory Response; GO:0050727, Regulation of Inflammatory Response; GO:0106014, Regulation of Inflammatory Response to Wounding; and GO:0002861, Regulation of Inflammatory Response to Antigenic Stimulus). ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal