Low-dose aspirin treatment reversed accelerated arterial thrombosis and increased platelet activity in Jak2^VF CH. (A) Experimental design of aspirin treatment (in the drinking water at 30 mg/L) in MK lineage–specific Jak2^VF. (B) Platelet activation as measured by P-selectin surface expression (B) and GPIIbIIIa activation (JON/A, C) of collagen- or thrombin-stimulated platelets of VF-Gp1ba mice after treatment with low-dose aspirin. (D) FeCl3-induced carotid artery thrombotic occlusion time (seconds) and (E) bleeding time in VF-Gp1ba mice or controls. (F) Experimental setup and quantification of FeCl₃-induced carotid artery thrombotic occlusion time (seconds) after treatment with low-dose aspirin (in the drinking water at 30 mg/L) in Jak2^VF CH and controls. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; Student t test or Mann-Whitney U test or 1-way analysis of variance as appropriate.