Accelerated arterial thrombosis and increased platelet activity in VF-Gp1ba mice. (A) MK lineage–specific expression of Jak2VF mouse model. (B) Platelet count (left), MPV (middle) and young platelet fraction (right). (C-F) Differential peripheral WBC count and hematocrit. (G-H) Platelet activation as measured by P-selectin surface expression (G) and GPIIbIIIa activation (JON/A, H) level of whole blood platelets at baseline or stimulated with platelet agonists ADP (10 μM), collagen (0.5 μM) or thrombin (0.5 U/mL). (I) Representative immunofluorescence-stained fluorescein isothiocyanate–labeled phalloidin images. Adhesion of platelets to fibrinogen-coated chamber slides with or without thrombin stimulation (0.5 U/mL). Scale bar, 5 μm. (J) FeCl3-induced carotid artery thrombotic occlusion time (seconds). Representative hematoxylin and eosin staining of thrombi were showed. Scale bar, 100 μm. (K) Tail bleeding time (seconds). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; Student t test or Mann-Whitney U test as appropriate.
