Figure 3.
Both PDS and mannose moieties drive p(Man-TLR7-PDS) tissue biodistribution. (A-D) Mice bearing day 18 C1498 were injected IV with fluorescently–labeled polymer variants (n = 3). After 8 hours, mice were sacrificed and organs were harvested, weighed, and homogenized to determine polymer distribution. Normalized fluorescence intensity values are given for (A) liver, (B) kidney, (C) spleen, and (D) plasma. (E) In a similar, separate experiment, mice were treated with fluorescent p(Man-TLR7-PDS) IV, with or without cytarabine pretreatment 1 day prior (n = 5). Normalized fluorescence of each tissue is given. (F) Fluorescence images of tissue sections showing polymer accumulation (red) and DAPI (gray) (scale bar, 400 μm). All data are plotted as mean ± SEM. Statistical analyses were performed using ordinary 1-way analysis of variance with multiple comparisons. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. DAPI, 4′,6-diamidino-2-phenylindole.

Both PDS and mannose moieties drive p(Man-TLR7-PDS) tissue biodistribution. (A-D) Mice bearing day 18 C1498 were injected IV with fluorescently–labeled polymer variants (n = 3). After 8 hours, mice were sacrificed and organs were harvested, weighed, and homogenized to determine polymer distribution. Normalized fluorescence intensity values are given for (A) liver, (B) kidney, (C) spleen, and (D) plasma. (E) In a similar, separate experiment, mice were treated with fluorescent p(Man-TLR7-PDS) IV, with or without cytarabine pretreatment 1 day prior (n = 5). Normalized fluorescence of each tissue is given. (F) Fluorescence images of tissue sections showing polymer accumulation (red) and DAPI (gray) (scale bar, 400 μm). All data are plotted as mean ± SEM. Statistical analyses were performed using ordinary 1-way analysis of variance with multiple comparisons. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. DAPI, 4′,6-diamidino-2-phenylindole.

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