Fgl1^−/− mice exhibit a blunted response to phlebotomy. (A) Fgl1 mRNA expression in the liver of male WT mice 36 hours after bleeding compared to control mice. Red blood cell count (RBC) (B) and hemoglobin (Hb) (C); Erfe mRNA expression in the bone marrow (D) and the spleen (E); and liver Hamp (F), Id1 (G), and Smad7 (H) mRNA expression in WT and Fgl1^−/− mice at t = 0 or 36 hours after phlebotomy. Data shown are mean ± SEM (n = 5-8) and were compared between control and phlebotomized mice using the Student t test (A) or between WT and Fgl1^−/− mice by 2-way ANOVA and corrected for multiple comparisons using the Holm-Šidák method (B-H). ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.