Fgl1 mRNA expression is induced by hypoxia.Hamp (A) and Fgl1 (B) mRNA expression in the liver, and Erfe and Fgl1 (C) mRNA expression in the bone marrow of 7-week-old mice at t = 0 to 20 hours after a single intraperitoneal injection of EPO (200 U; n = 5). (D) Fgl1 mRNA expression in the liver of 8-week-old WT, Th3/+, and Erfe^−/−; Th3/+ mice (n = 7-9). (E) Fold change of Fgl1 mRNA expression in mouse primary hepatocytes in serum-free media and incubated for 15 hours in low-oxygen condition (2%) or in presence of prolyl-hydroxylases inhibitor DMOG compared with untreated cells. (F) Fgl1 mRNA expression in the liver of Vhl-deficient mice (Albumin-Cre/VHL^flox/flox), and (G) mice treated with prolyl-hydroxylase inhibitor vadadustat. Data shown are mean ± SEM and were compared for each time point with values for control mice at t = 0 (A-C) or to WT mice (D) by 1-way ANOVA and were corrected for multiple comparisons by the Holm-Šidák method or with WT or vehicle-treated mice by the Student t test (F-G). Data shown for the experiment in primary hepatocytes are means of 3 independent experiments and were compared with control cells by the Student t test (E). ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.